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	Provedor de dados J. Venom. Anim. Toxins (3) Autor GIGLIO,J. R. (3) SOARES,A. M. (2) ANZALONI PEDROSA,L. H. (1) BATINA,M. de F. C. (1) BORJA-OLIVEIRA,C. R. (1) DA SILVA,R. J. (1) FONTES,M. R. M. (1) HYSLOP,S. (1) PRADO-FRANCESCHI,J. (1) RODRIGUES-SIMIONI,L. (1) SAMPAIO,S. V. (1) ZAMUNÉR,S. R. (1) Mais... Palavra-chave Basic toxins (1) Bothrops neuwiedii venom (1) Crotalus durissus terrificus (1) Elapidae (1) Intraspecific variation (1) LD50 (1) Myotoxicity (1) Neuromuscular blockade (1) Neurotoxicity (1) Peschiera fuchsiaefolia (1) Polyacrylamide gel electrophoresis (1) Snake venom antidotes (1) Snake venoms (1) Viperidae (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (1) Ano 2002 (1) 1998 (1) 1997 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última INTRASPECIFIC VARIATION IN NEUROTOXIC AND MYOTOXIC ACTIVITIES OF Bothrops neuwiedii VENOMS J. Venom. Anim. Toxins BORJA-OLIVEIRA,C. R.; SOARES,A. M.; ZAMUNÉR,S. R.; HYSLOP,S.; GIGLIO,J. R.; PRADO-FRANCESCHI,J.; RODRIGUES-SIMIONI,L.. Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops neuwiedii venom; Intraspecific variation; Myotoxicity; Neurotoxicity; Neuromuscular blockade. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100008 METHODOLOGICAL CARE IN THE EVALUATION OF THE LD50 AND OF THE NEUTRALIZATION OF THE LETHAL EFFECT OF Crotalus durissus terrificus VENOM BY THE PLANT Peschiera fuchsiaefolia (APOCYNACEAE) J. Venom. Anim. Toxins BATINA,M. de F. C.; GIGLIO,J. R.; SAMPAIO,S. V.. In the present study, we demonstrate that the volumes in which a given protein mass of Crotalus durissus terrificus venom or of a lyophilized stabilized aqueous extract (LSAE) of Peschiera fuchsiaefolia, an antivenom agent injected intramuscularly, have a decisive influence on the results. The LD50 of C. d. terrificus venom injected i.m. in a final volume of 200µl (2µl/g) (saline solution, 0.9% NaCl) was 180µg/100g rat body weight (p<0.05, 161 to 202µg/100g body weight) and the LD50 of the venom injected i.m. in a volume of 50µl (0.5µl/g) was 120µg/100g body weight (p<0.05, 107 to 134 µg/100g body weight). The reduction of the final volume injected i.m. also required a reduced mass of LSAE necessary to neutralize the lethal effect of C. d.... Tipo: Info:eu-repo/semantics/article Palavras-chave: LD50; Peschiera fuchsiaefolia; Crotalus durissus terrificus; Snake venom antidotes. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000100003 POLYACRYLAMIDE GEL ELECTROPHORESIS AS A TOOL FOR THE TAXONOMIC IDENTIFICATION OF SNAKES FROM THE ELAPIDAE AND VIPERIDAE FAMILIES J. Venom. Anim. Toxins SOARES,A. M.; ANZALONI PEDROSA,L. H.; FONTES,M. R. M.; DA SILVA,R. J.; GIGLIO,J. R.. Polyacrylamide gel electrophoresis (PAGE) for basic proteins may be a useful tool for the characterization of whole snake venoms and for the taxonomic classification of snakes of the Elapidae and Viperidae families. However, due to the close proximity of molecular weights among toxins bearing different net charges, sodium dodecyl sulfate (SDS) - PAGE was not able to provide an efficient differentiation. This article reports the electrophoretic analysis of several venoms from the genera Micrurus, Bothrops, Bothriopsis, Crotalus and Lachesis and shows a typical and distinctive electrophoretic profile for each species, with intraspecific and geographic variations. Even in cases in which extreme morphological similarities were present, such as between B.... Tipo: Info:eu-repo/semantics/other Palavras-chave: Snake venoms; Basic toxins; Polyacrylamide gel electrophoresis; Elapidae; Viperidae. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000200005 Registros recuperados: 3 Primeira ... 1 ... Última

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